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About The Other Measurements

Overview: Cholesterol is a lipid in blood that helps with normal cell function within an individual. This substance is produced within the body and helps with the production of vitamin D, bile salts, and hormones. Cholesterol comes in two well known forms: LDL and HDL. LDL is typically called the bad cholesterol because it can raise your risk of heart disease and stroke. HDL, the good cholesterol, is linked to the lowering risk of heart disease and stroke. Other factors such as family history, age, genetics, and more can lead to a greater chance for chronic diseases such as heart disease.

Measurement: Cholesterol has been formally tested with a simple blood test, which analyzes all types of fat within the blood including total,LDL, and HDL cholesterol. The most common known test is called the Vertical Auto Profile test, which measures total cholesterol, LDL,HDL,VLDL,IDL, and Triglycerides.

With this information, the prevention of cardiovascular disease is at the forefront of helping those individuals in need.The steps to actually measuring the blood for cholesterol include various coupling reactions that ultimately measure the color intensity. The specific steps for each type of cholesterol tested is as follows:

Total Cholesterol: Cholesterol is measured enzymatically in serum or plasma in a series of coupled reactions that hydrolyze cholesteryl esters and oxidize the 3-OH group of cholesterol. One of the reaction byproducts, H2O2 is measured quantitatively in a peroxidase catalyzed reaction that produces a color. Absorbance is measured at 500 nm. The color intensity is proportional to cholesterol concentration.(1)

Triglycerides: Triglycerides are measured enzymatically in serum or plasma using a series of coupled reactions in which triglycerides are hydrolyzed to produce glycerol. Glycerol is then oxidized using glycerol oxidase, and H2O2, one of the reaction products, is measured as described above for cholesterol. Absorbance is measured at 500 nm.(1)

HDL: HDL is measured directly in serum. The basic method is that apoB containing lipoproteins in the specimen react with a blocking reagent that renders them non-reactive with the enzymatic cholesterol reagent under conditions of the assay. The apoB containing lipoproteins are thus effectively excluded from the assay and only HDL-chol is detected under the assay conditions.(1)

LDL: The understanding of how LDL is measured has changed significantaly in the past couple years. There are two ways that LDL can be measured. The first way is to calculate measured values of total cholesterol, triglycerides and HDLcholesterol according to the relationship: [LDL-chol] = [total chol] – [HDL-chol] – [TG]/5 where [TG]/5 is an estimate of VLDL-cholesterol and all values are expressed in mg/dL. The second known way is to undergo a direct LDD test due to unusually high triglyceride levels that allow for a poor calculation using the formula above.

Glucose monitoring has been an important tool for diabetics for years, but recent developments in constant monitoring technologies have total health implications as well. Tracking dips and spikes in a person’s blood sugar provides real-time feedback on their insulin sensitivity, which is a key indicator of metabolic health.

Even for non-diabetics tracking your insulin response to outside influences such as diet or exercise and learning how to maintain a steady blood-sugar level is key to establishing a solid foundation for overall health. While it serves as a secondary function, the MetaView chip also tracks the user’s insulin levels, providing real-time feedback through the app, and creating the most comprehensive measure of overall metabolic health yet.

  1. http://www.cdc.gov/nchs/data/nhanes/nhanes_03_04/l13_c_met_lipids.pdf

 

1 Comment

  1. Michael Johnston

    What a thorough analysis of measuring cholestorol! Very informative!

    Reply

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